Journal: Cell reports

Article Title: Functional genomics identifies N -acetyllactosamine extension of complex N -glycans as a mechanism to evade lysis by natural killer cells

doi: 10.1016/j.celrep.2024.114105

Figure Lengend Snippet: KEY RESOURCES TABLE

Article Snippet: Dead Cell Removal Kit , Miltenyi Biotec , 130-090-101.

Techniques: Virus, Cell Isolation, Recombinant, Plasmid Preparation, Selection, CRISPR, Glycoproteomics, Knock-Out, Software

Journal: Acta Endocrinologica (Bucharest)

Article Title: EVALUATION OF FETUIN-A LEVELS IN THE EARLY STAGE OF AUTOIMMUNE THYROIDITIS

doi: 10.4183/aeb.2023.301

Figure Lengend Snippet: Evaluation of the performance of fetuin-A in predicting Hashimoto’s thyroiditis using ROC curve analysis

Article Snippet: Serum fetuin-A levels were determined using a human fetuin-A ELISA kit (Elabscience Biotechnology, Houston, TX, USA).

Techniques:

Journal: Nanomaterials

Article Title: Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

doi: 10.3390/nano12030345

Figure Lengend Snippet: ELISA kits used to quantify the different ECM components.

Article Snippet: LAMB1 , Porcine Laminin subunit beta-1 (LAMB1) ELISA Kit (MyBioSource, San Diego, CA, USA) , MBS2613645.

Techniques: Enzyme-linked Immunosorbent Assay, Collagen Assay

Journal: Nanomaterials

Article Title: Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

doi: 10.3390/nano12030345

Figure Lengend Snippet: List of primers used for quantitative PCR analysis.

Article Snippet: LAMB1 , Porcine Laminin subunit beta-1 (LAMB1) ELISA Kit (MyBioSource, San Diego, CA, USA) , MBS2613645.

Techniques: Real-time Polymerase Chain Reaction

Journal: Nanomaterials

Article Title: Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

doi: 10.3390/nano12030345

Figure Lengend Snippet: Histochemical and immunohistochemical analysis, stereological quantification, and ELISA tests in young and aged ovaries. ( a ) Masson’s trichrome staining showed the presence of collagen (blue) and elastic (magenta) fibers in both young and aged ovaries. Scale bars = 100 μm; ( b ) Stereological analysis demonstrated a significant increase of collagen fibers in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( c ) ELISA quantifications indicated a significant increase of the collagen content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( d ) Gomori’s Aldehyde Fuchsin staining revealed the presence of elastic fibers in both young and aged ovaries. Scale bars = 100 μm; ( e ) Stereological analysis displayed a significant decrease of elastic fibers in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( f ) ELISA quantifications confirmed a significant decrease of the elastin content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( g ) Alcian blue staining showed the presence of GAGs in both young and aged ovaries. Scale bars = 100 μm; ( h ) Stereological analysis indicated a significant increase of GAGs in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), * p < 0.05; ( i ) ELISA quantifications confirmed a significant increase of the GAG content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), * p < 0.05; ( j ) Immunohistochemical staining of young and aged ovaries for LAMA3. Scale bars = 100 μm; ( k ) Stereological analysis demonstrated a significant increase of LAMA3 in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( l ) ELISA quantifications showed a significant increase of the LAMA3 content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( m ) Immunohistochemical staining of young and aged ovaries for LAMB1. Scale bars = 100 μm; ( n ) Stereological analysis revealed a significant increase of LAMB1 in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( o ) ELISA quantifications displayed a significant increase of the LAMB1 content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( p ) Immunohistochemical staining of young and aged ovaries for FN1. Scale bars = 100 μm; ( q ) Stereological analysis indicated a significant decrease in FN1 content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( r ) ELISA quantifications showed a significant increase of the LAMB1 content in aged ovaries compared to the young ones. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01.

Article Snippet: LAMB1 , Porcine Laminin subunit beta-1 (LAMB1) ELISA Kit (MyBioSource, San Diego, CA, USA) , MBS2613645.

Techniques: Immunohistochemical staining, Enzyme-linked Immunosorbent Assay, Staining

Journal: Nanomaterials

Article Title: Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

doi: 10.3390/nano12030345

Figure Lengend Snippet: Gene expression changes of extracellular matrix-related genes in young (white bars) and aged ovaries (black bars). Expression levels of collagens (COL1A1, COL3A1, COL4A2), elastin (ELN), fibronectin (FN1), glycoprotein (EMILIN1), laminins (LAMA3, LAMB1), proteoglycans (VCAN, HSPG2, CSPG4), and proteases (ELANE, MMP1, MMP2, MMP3, MMP9, MMP12, and MMP14). Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), * p < 0.05, ** p < 0.01, *** p < 0.001.

Article Snippet: LAMB1 , Porcine Laminin subunit beta-1 (LAMB1) ELISA Kit (MyBioSource, San Diego, CA, USA) , MBS2613645.

Techniques: Expressing

Journal: Nanomaterials

Article Title: Impact of Aging on the Ovarian Extracellular Matrix and Derived 3D Scaffolds

doi: 10.3390/nano12030345

Figure Lengend Snippet: Immunohistochemical characterizations of young and aged ECM-based scaffolds. ( a ) LAMA3 immunostaining displayed the persistence of the fibers after decellularization in both young and aged tissues; Stereological analysis ( b ) and ELISA quantifications (c) demonstrated no significant differences between native (Native) and decellularized (Scaffold) ovaries in LAMA3 content. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( d ) LAMB1 immunostaining showed the maintenance of the fibers at the end of the process in both young and aged organs; Stereological analysis ( e ) and ELISA tests ( f ) indicated no significant differences between native (Native) and decellularized tissues (Scaffold) in LAMB1 content. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01; ( g ) FN1 immunostaining revealed fiber retainment in both young and aged decellularized tissues; Stereological ( h ) and ELISA analysis ( i ) showed no significant differences between native (Native) and decellularized samples (Scaffold) in FN1 content. Data are expressed as the mean. Error bars represent the standard error of the mean (SEM), ** p < 0.01.

Article Snippet: LAMB1 , Porcine Laminin subunit beta-1 (LAMB1) ELISA Kit (MyBioSource, San Diego, CA, USA) , MBS2613645.

Techniques: Immunohistochemical staining, Immunostaining, Enzyme-linked Immunosorbent Assay

Journal: Frontiers in Immunology

Article Title: Allergic T H 2 Response Governed by B-Cell Lymphoma 6 Function in Naturally Occurring Memory Phenotype CD4 + T Cells

doi: 10.3389/fimmu.2018.00750

Figure Lengend Snippet: Role of Bcl6-mediated MPT cell functions in NAT H 2 differentiation in an allergic murine model. [ (A) top] Mixture of purified KJ1-26 − MPT cells ( Bcl6 -WT or Bcl6 -KO) and KJ1-26 + WT naïve CD4 + T cells were transferred into BALB/c nu/nu mice intravenously (day 0). These mice were immunized with alum-conjugated OVA and then intratracheally challenged with OVA. [ (A) bottom] Absolute cell numbers of Neu, Eos, AM, and Lym in BALF, (B) hematoxylin and eosin-stained, formalin-fixed lung sections (magnification: 200×), and (C) T H 2 cytokine levels in the BALF of recipient mice 48 h after the last OVA challenge. (D) Relative Il4, Il5 , and Il13 expression mRNA in splenic KJ1-26 + T cells restimulated with anti-CD3 monoclonal antibodies 5 days after the last challenge. (E) OVA-specific IgE antibody titers in sera from each recipient of Bcl6 -WT NAT H 2 cells, plus MPT H 2 cells transferred from Bcl6 -TG, Bcl6 -WT, or Bcl6 -KO mice 2 days after the last challenge. All results are representative of four independent experiments with similar outcomes. Data are presented as the mean ± SEM ( n = 5–7). * P < 0.05, ** P < 0.01, comparison between two groups is indicated. AM, alveolar macrophages; BALF, bronchoalveolar lavage fluid; Bcl6, B-cell lymphoma 6; Eos, eosinophils; KJ + , KJ1-26-positive; KO, knockout; Lym, lymphocytes; MPT cell, memory phenotype CD4 + T cell; MPT H 2 cell, MPT cell-derived T H 2 cell; NAT H 2 cell; naïve CD4 + T cell-derived T H 2 cell; Neu, neutrophils; NS, not significant; OVA, ovalbumin; TG, transgenic; WT, wild-type.

Article Snippet: IgE anti-OVA Abs were detected using a mouse anti-OVA IgE Antibody Assay Kit (Chondrex, Redmond, WA, USA).

Techniques: Purification, Staining, Expressing, Knock-Out, Derivative Assay, Transgenic Assay

Journal: BBA Clinical

Article Title: Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients

doi: 10.1016/j.bbacli.2017.05.003

Figure Lengend Snippet: Label-free mass spectrometry data. List of statistically significant discovered proteins using LC-MS analysis. Proteins (in bold) were selected for further validation using ELISAs.

Article Snippet: Four commercially available kits for these four proteins; angiogenin (ANG) [Abcam, UK - ab99970], clusterin (CLU) [R&D system, UK - DCLU00], C-C Motif Chemokine 18 (CCL18) [Abcam, UK - ab100620], and Complement C1q [Abcam, UK - ab170246] were used.

Techniques: Mass Spectrometry, Biomarker Discovery, Variant Assay

Journal: BBA Clinical

Article Title: Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients

doi: 10.1016/j.bbacli.2017.05.003

Figure Lengend Snippet: ELISA Data. Mean, SD, Area under the curve (AUC) and p -value for each of the new and standard proteins found in the two groups of patients compared.

Article Snippet: Four commercially available kits for these four proteins; angiogenin (ANG) [Abcam, UK - ab99970], clusterin (CLU) [R&D system, UK - DCLU00], C-C Motif Chemokine 18 (CCL18) [Abcam, UK - ab100620], and Complement C1q [Abcam, UK - ab170246] were used.

Techniques: Enzyme-linked Immunosorbent Assay

Journal: BBA Clinical

Article Title: Novel panel of protein biomarkers to predict response to bortezomib-containing induction regimens in multiple myeloma patients

doi: 10.1016/j.bbacli.2017.05.003

Figure Lengend Snippet: Logistic regression analysis data. List of different protein combinations used to establish the best model that can be used as a predictive panel for response to induction therapy containing bortezomib regime.

Article Snippet: Four commercially available kits for these four proteins; angiogenin (ANG) [Abcam, UK - ab99970], clusterin (CLU) [R&D system, UK - DCLU00], C-C Motif Chemokine 18 (CCL18) [Abcam, UK - ab100620], and Complement C1q [Abcam, UK - ab170246] were used.

Techniques: